Phytotoxicity of trihalomethanes and trichloroacetic acid on Vigna radiata and Allium cepa plant models.

Disinfection by-products (DBPs) are a concern due to their presence in chlorinated wastewater, sewage treatment plant discharge, and surface water, and their potential for environmental toxicity. Despite some attention to their ecotoxicity, little is known about the phytotoxicity of DBPs. This study aimed to evaluate the individual and combined phytotoxicity of four trihalomethanes (THMs: trichloromethane (TCM), bromodichloromethane (BDCM), dibromochloromethane (DBCM), and tribromomethane (TBM) and their mixture (THM4)), and trichloroacetic acid (TCAA) using genotoxic and cytotoxic assays. The analysis included seed germination tests using Vigna radiata and root growth tests, mitosis studies, oxidative stress response, chromosomal aberrations (CA), and DNA laddering using Allium cepa. The results showed a progressive increase in root growth inhibition for both plant species as the concentration of DBPs increased. High concentrations of mixtures of four THMs resulted in significant (p < 0.05) antagonistic interactions. The effective concentration (EC50) value for V. radiata was 5655, 3145, 2690, 1465, 3570, and 725 mg/L for TCM, BDCM, DBCM, TBM, THM4, and TCAA, respectively. For A. cepa, the EC50 for the same contaminants was 700, 400, 350, 250, 450, and 105 mg/L, respectively. DBP cytotoxicity was observed through CAs, including C-metaphase, unseparated anaphase, lagging chromosome, sticky metaphase, and bridging. Mitotic depression (MD) increased with dose, reaching up to 54.4% for TCAA (50-500 mg/L). The electrophoresis assay showed DNA fragmentation and shearing, suggesting genotoxicity for some DBPs. The order of phytotoxicity for the tested DBPs was TCAA > TBM > DBCM > BDCM > THM4 > TCM. These findings underscore the need for further research on the phytotoxicity of DBPs, especially given their common use in agricultural practices such as irrigation and the use of sludge as manure.

Determination of ignition temperature of municipal solid waste for understanding surface and sub-surface landfill fire.

Open dumping of municipal solid waste (MSW) is a common practice in India which leads to a number of problems like non-uniform compaction, slope failure, percolation of water during rainy seasons, abrupt leachate generation and stability issues. It also leads to various other issues, such as manual scavenging and deliberate waste burning. During the waste degradation process, an enormous amount of heat and landfill gases (LFGs) like carbon dioxide (CO2) and methane (CH4) are generated. Biological and chemical reactions happening at the surface and inside the landfill contribute to the heat generation. Initiation of waste ignition is a major cause of spontaneous landfill fire. The risk posed by landfill fires is high in India as most of the landfills are non-engineered. The present study aims to determine the ignition temperature of waste dumped at Bhandewadi dumpsite located at Nagpur, India which will enable a better understanding of the initiation of fires in non-engineered landfill (surface and sub-surface fires), especially in Indian condition. The results of the present study showed that ignition temperature is directly proportional to the moisture content of MSW for its values ranging from 5 to 55% by mass. The correlation of smoldering time (Ts) and ignition time (Ti) of MSW with its age under gradual temperature rise in a muffle furnace (i.e., 3 °C/min) were also studied and it was found that Ts and Ti are directly proportional to the age of MSW and the values ranged from 23 to 34 min for Ts and 27 to 48 min for Ti.